Thermal stability and nuclease-resistance properties of oligonucleotides having an aminoalkyl side chain at the nucleobase and sugar moieties
نویسندگان
چکیده
Recent studies concerning properties of oligonucleotides (ONs) with aminoalkyl side chains at the nucleobase and sugar moieties have been comprehensively reviewed in relation to the antisense, antigene, ribozyme, aptamer, decoy, and siRNA strategies. Thermal stabilities of ONs incorporating nucleoside analogs with a wide variety of aminoalkyl side chains at the 5and 4-positions of pyrimidine bases, the 2-position of a purine base, the 1’-, 2’-, 4’and 5’-positions of sugar moieties, and the 6’-position of a carbocyclic nucleoside have been compared. The resistant properties of these ONs to hydrolysis by endoand exo-nucleases are also discussed.
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